ISOLATION OF A TENFOLD HIS-TAGGED D1 PROTEIN FROM CHLAMYDOMONAS REINHARDTII USING NI-NTA AGAROSE

Ilka HÄNßGEN

Анотація


Щоб зробити білок D1 доступним для дослідження та аналізу змін, чистий препарат D1, який здійснює накопичення білка D1 швидким та відтворювальним методом, є необхідним. Дана робота представляє цей метод. Внутрішня структура білка D1 chlorophycee Chlamydomonas reinhardtii була включена в цикл D-e. Після зв’язування рідної, солюбілізованої фотосистеми ІІ (ФС) для Ni-NTA агарози білка D1 зібрані компоненти були змиті за допомогою 8 М мочевини та 0,3 % SDS. Ізольовані білки D1 можна виявити за допомогою фарбування в Кумассі після SDSPAGE. Вимивається білок D1, який міститься у вигляді домішки тільки білка D2, який був зв’язаний з D1. Використання перекису водню показали, що очищений білок D1 може бути використаний для обмеження аналізу в пробірці. Обмежені фрагменти легко виявити на срібному забарвленні – замість реакції антитіл.

Ключові слова


Chlamydomonas reinhardtii; білок D1; фотосистема II; His-tag

Повний текст:

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Посилання


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